Biotech Studies 2016, Vol 25, Num, 1     (Pages: 052-057)

Determination of Haploid Induction Rates of Different Inducer Lines Used for In-Vivo Double Haploid Technique in Hybrid Maize Breeding

İbrahim CERİT 1 ,Gönül CÖMERTPAY 1 ,Rüstem OYUCU 1 ,Bülent ÇAKIR 1 ,Rüştü HATİPOĞLU 2 ,Hakan ÖZKAN 2

1 Doğu Akdeniz Tarımsal Araştırma Enstitüsü Müdürlüğü, Adana
2 Çukurova Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü, Adana
DOI : 10.21566/tarbitderg.280162 - This research was conducted to determine haploid induction rate (HIR) of different inducer lines used for in-vivo double haploid technique in hybrid maize breeding at the East Mediterranean Agricultural Research Institute. In the study RWS, RWK-76 inducer lines, their cross RWS X RWK-76 and Stock-6 were used as male parent for haploid induction. In the study 75 different F2 genotypes as female parent were used for haploid seed production. The 66 of F2 genotypes were selected from the corn breeding material of East Mediterranean Agricultural Research Institute, and 9 genotypes were selected from open pollinated populations of the commercial hybrid cultivars. Results of the study showed that haploid induction rate of different inducer genotypes changed depending on the female parent. It was obtained 1463 haploid seeds from 69 F2 genotypes of total 75 F2 genotypes. The highest haploid induction rate (7.8%) was obtained from the inducer RWK-76 line, while the lowest haploid induction rate (1.28%) was obtained from the stock-6, and average haploid induction rate (HIR) was 4.79%. Keywords : Maize, breeding, inducer line, haploid