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Biotech Studies 2016, Vol 25, Num, 1     (Pages: 035-041)

In Vitro Propagation of Endemic `Kaba Navruz` Iris galatica Siehe

Satı UZUN 1 ,Ali İrfan İLBAŞ 2 ,Arif İPEK 3 ,Erman BEYZİ 1 ,Serkan URANBEY 4 ,Neşet ARSLAN 4

1 Erciyes Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Kayseri, Türkiye
2 Kyrgyzstan-Türkiye Manas Üniversitesi Ziraat Fakültesi, Bişkek, Kırgızistan
3 Çankırı Karatekin Üniversitesi, Fen Fakültesi, Biyoloji Bölümü Çankırı, Türkiye
4 Ankara Üniversitesi , Ziraat Fakültesi, Tarla Bitkileri Bölümü, Ankara, Türkiye
DOI : 10.21566/tbmaed.01228 Viewed : 579 - Downloaded : 120 In this study, a protocol for in vitro propagation of endemic Iris galatica species was developed. For this purpose, immature embryo and leaf explants of I. galatica were cultured on Murashige ve Skoog (MS) media supplemented with various concentrations and combination of thidiazuron (TDZ), and α-naphthalenacetic acid (NAA). 4.85 shoot per embryo and 3.04 shoot per leaf explant were obtained. 3-5 cm rejenerated shoots were rooted and the best medium for rooting was obtained from 1 mg/l IBA or 1 mg/l NAA. Keywords : Iris galatica, tissue culture, in vitro shoot regeneration, immature embryo, rooting