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¹ Istanbul Medipol University, School of Pharmacy, Department of Biochemistry, Goztepe District, Ataturk Street, No:40, 34810, Beykoz, İstanbul, Türkiye
² Istanbul Medipol University, School of Pharmacy, Department of Pharmacology, Goztepe District, Ataturk Street, No:40, 34810, Beykoz, Istanbul, Türkiye
³ Yeditepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Inönü District, Kayisdagi Street, 34755, Atasehir, Istanbul, Türkiye
⁴ University of Granada, Faculty of Science, 18071, Granada, Spain DOI : 10.38042/xx.xx - One of the significant sources of vitamins and minerals is herbal tea, which is most commonly analyzed using HPLC. The study aimed to develop a new, inexpensive, and an innovative method, rather than relying on expensive methods. The rope dyeing method starts with the preparation of two different herbal teas at specific concentrations and the determination of individual and nanocomposite forms of vitamin D3, vitamin C, and magnesium (Mg). After that, macramé strings are dipped in four different ink dyes in the sample. A certain pressure must be applied before pulling the strings, and the reaction on the sketch paper is photographed to proceed with color analysis using the Image J software. While the nanocomposite form of lemon tea has the highest concentration of vitamin C (46.01 ± 0.01 ppm), the individual form of aronia tea has the highest amount of vitamin D3 (49.03 ± 0.01 ppm). Additionally, the highest concentration of Mg was found in the nanocomposite extract of the aronia tea (78.47 ± 0.05 ppm). This consistency between methods reinforces the reliability of the analytical approach, as the HPLC results showed a 96.8% correlation with the rope dyeing method, validating the accuracy and reproducibility of the findings. Keywords : Herbal tea, Montmorillonite clay nanocomposite, Rope dyeing method, Vitamin C, Vitamin D3