Abstract Full Text:PDF Recommended articles Mail to Author How to Cite

¹ Graduate School of Natural and Applied Sciences, Department of Bioengineering, Ege University, 35040 Bornova, Izmir, Türkiye
² Faculty of Engineering, Department of Bioengineering, Ege University, 35040 Bornova, Izmir, Türkiye DOI : 10.38042/biotechstudies.2023225 - Indole-3-acetic acid is an important phytohormone synthesized by plant growth-promoting rhizobacteria (PGPR), widely used in agriculture due to its various abilities. This study aimed to develop an economical PGPR-based nano-bioformulation with high Indole-3-acetic acid production capacity. In this context, Bacillus subtilis 4-Ka-22 was used, and initial optimization of the growth medium was carried out. Subsequently, iron salts (FeSO₄ and FeCl₃) were added separately at different concentrations and under varying conditions, both before and after bacterial cultivation. Changes in Indole-3-acetic acid levels were evaluated along with the antagonistic activity and protease enzyme production. In addition, the potential of iron salt addition to induce iron-based nanomaterial/nanoparticle formation was investigated. According to the results, the addition of 15 mM FeSO₄ after cultivation, followed by incubation at 68°C and pH 13 with stirring at 300 rpm for 48 h, led to a 9.15-fold increase in Indole-3-acetic acid production in the B. subtilis 4-Ka-22 culture broth, compared to the control group. These findings suggest that the methodology applied in this study could be adapted to other bacterial strains with similar biosynthetic pathways to enhance Indole-3-acetic acid production. In this regard, the study provides a valuable foundation for the development of PGPR-based nano-biotechnological applications. Keywords : Bacillus subtilis, Indole-3-acetic acid (IAA), Magnetic iron nanomaterials, Nano-bioformulation