Biotech Studies 2017, Vol 26, Num, 1     (Pages: 001-010)

Effect of Different Sowing Times on Leaf Characteristics and Indican Content of Some Isatis Species

Nazan ÇÖMLEKCİOĞLU 1 ,Lale EFE 2 ,Şengül KARAMAN 1

1 Biology Department, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Türkiye
2 Field Crops Department, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Türkiye
DOI : 10.21566/tarbitderg.323555 - In this study, effects of different sowing dates on the some agronomic properties of 4 Isatis species (Isatis tinctoria L., Isatis candolleana BOISS. (endemic), Isatis tinctoria L. subsp. corymbosa (BOISS.) DAVIS and Isatis buschiana SCHISCHKIN were investigated in ecological conditions of Kahramanmaras in 2008 and 2009 growing season. Optimum sowing time for I. tinctoria leaf yield was spring and for the other species were autumn. The highest leaf yield was obtained in 2008 for all species and the values were 15185 kg ha-1 in I. tinctoria, 15109 kg ha-1, 19299 and 10909 kg ha-1 in I. tinctoria subsp. corymbosa, I. buschiana and I. candolleana, respectively. The highest indican contents were found 23.5, 21.4, 18.2 and 15.2 mg g-1 in I. buschiana, I. tinctoria subsp. corymbosa, I. tinctoria and I. candolleana respectively, by using ultrasonic extraction and DAD detector analysis of HPLC. Keywords : HPLC-DAD, Isatis, indican, leaf yield, sowing date