Biotech Studies 2016, Vol 25, Num, 2     (Pages: 088-093)

Determination of Appropirate Gamma Ray Dose and Effect on Seedlig Growth in M1 of Different Gamma Ray Dose in Cotton (Gossypium hirsitum L.) Variety Nazilli 663.

Levent YAZICI 1 ,Süleyman ÇİÇEK 2 ,Fatih KÜÇÜKTABAN 2 ,Mehmet ÇOBAN 2 ,Nina TUNCEL 3

1 Orta Karadeniz Geçit Kuşağı Tarımsal Araştırma Enstitüsü Müdürlüğü, Tokat
2 Pamuk Araştırma Enstitüsü Müdürlüğü, Aydın
3 Akdeniz Üniversitesi, Fen Fakültesi, Fizik Bölümü, Antalya
DOI : 10.21566/tarbitderg.281862 Viewed : 440 - Downloaded : 298 In this study, Different gamma ray radiation dose were applied to Nazilli 663 cotton varieties seeds for determine their effects on some plant characteristics in M1 plants and the optimal gamma rays effective mutation dose values in mutation breeding work. Nazilli 663 seeds of cotton varieties were applied gamma irradiation in 8 different doses (0, 150, 250, 350, 450, 550, 650 and 750). Output rate (%), seedling height (cm), seedling root length (cm), seedling dry weight (g), seedling hypocotyl length (cm), seedling epicotyl length (cm) and seedling fresh weight (g), effects were investigated in M1 plants. Research was carried out at room climate-controlled conditions of Nazilli Cotton Research Institute. Gamma rays were applied to seeds from Akdeniz University Physics Department Cobalt 60 source. The trial was conducted as a randomized complete block experimental design in three replications. As a result, as parallel to increasing doses applied of gamma-ray dose s seedling height (cm), seedling root length (cm), seedling dry weight (g) adversely were affected in Nazilli 663 cotton varieties. 150 gray'lik dose seedling hypocotyl length effect and output ratio of 150 and 250 Gray'lik doses was higher appear than control plants adversely affected higher doses output rate and seedling hypocotyl length. On the other hand `Effective Mutation Dose ` (ED50) is calculated by linear regression analysis, 309 Gy dose was determined as effective dose of mutations that it is 50% reduce dose of seedling length. Keywords : Nazilli 663, mutation, M1 plants, gamma radiation, effective mutations dose